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Photosynthesis in Higher Plants

45 NEET-UG practice questions on Photosynthesis in Higher Plants, part of Botany. The first 25 are below; the first 5 with answers and explanations (tap Show answer after you have chosen), the rest in Learn with a free account.

  1. Question 1 · Medium

    A leaf makes three molecules of glucose through the Calvin cycle. How much ATP and NADPH does this require in all?

    1. A18 ATP and 12 NADPH
    2. B54 ATP and 18 NADPH
    3. C54 ATP and 36 NADPH
    4. D36 ATP and 54 NADPH
    Show answer

    Answer: C · 54 ATP and 36 NADPH

    Fixing one CO₂ needs 3 ATP and 2 NADPH, and one glucose needs six turns, so one glucose uses 18 ATP and 12 NADPH. Three glucose molecules need 3 × 18 = 54 ATP and 3 × 12 = 36 NADPH. Check: 18 CO₂ × 3 = 54 and 18 CO₂ × 2 = 36.

    • A: This is the need for only one glucose.
    • B: The ATP is right, but NADPH was counted as one per CO₂.
    • D: This swaps the ATP and NADPH figures.

    Remember this

    Per CO₂ fixed, 3 ATP and 2 NADPH; per glucose, 6 turns, 18 ATP and 12 NADPH.

  2. Question 2 · Medium

    Assertion A: Photorespiration is a wasteful process in C3 plants. Reason R: In photorespiration, RuBisCO binds O₂, no sugar, ATP or NADPH is formed, and CO₂ is released with the use of ATP. Choose the correct answer.

    1. AA is correct but R is not correct
    2. BBoth A and R are correct and R is the correct explanation of A
    3. CA is not correct but R is correct
    4. DBoth A and R are correct but R is not the correct explanation of A
    Show answer

    Answer: B · Both A and R are correct and R is the correct explanation of A

    When RuBisCO binds O₂, RuBP forms one 3-phosphoglyceric acid and one phosphoglycolate. The pathway makes no sugar, ATP or NADPH and even spends ATP while releasing CO₂. That is exactly why it is wasteful.

    • A: R is a correct account of photorespiration.
    • C: A is correct, as NCERT calls the pathway wasteful.
    • D: R directly states why the process is wasteful.

    Remember this

    Photorespiration occurs in C3 plants, needs O₂ at RuBisCO, makes nothing useful, and is absent in C4 plants.

  3. Question 3 · Easy

    Priestley kept a mint plant in a closed bell jar with a burning candle, and with a mouse in another trial. Which conclusion did he draw?

    1. APlants release the CO₂ that a candle needs to keep burning
    2. BPlants purify the air only when they are placed in sunlight
    3. CPlants restore what candles and breathing animals remove from air
    4. DGreen plant parts store the glucose they make as starch
    Show answer

    Answer: C · Plants restore what candles and breathing animals remove from air

    The candle went out and the mouse died in a closed jar without a plant, but both continued when a mint plant was present. Priestley inferred that plants restore something to the air that burning and breathing use up.

    • A: Candles need oxygen, not CO₂, so this reverses the gas involved.
    • B: The need for sunlight was shown later by Ingenhousz.
    • D: This was the finding of Julius von Sachs.

    Remember this

    Priestley showed plants restore air; Ingenhousz added the need for sunlight; Sachs showed glucose and starch formation.

  4. Question 4 · Easy

    Within the mesophyll cells of maize, which molecule is the primary acceptor of CO₂?

    1. APhosphoenol pyruvate
    2. BRibulose bisphosphate
    3. C3-phosphoglyceric acid
    4. DOxaloacetic acid
    Show answer

    Answer: A · Phosphoenol pyruvate

    Maize is a C4 plant. Its mesophyll cells fix CO₂ onto the 3-carbon molecule phosphoenol pyruvate using PEP carboxylase, which forms the 4-carbon oxaloacetic acid.

    • B: RuBP accepts CO₂ in the Calvin cycle, which runs in the bundle sheath of maize.
    • C: 3-phosphoglyceric acid is the first product of the C3 pathway.
    • D: Oxaloacetic acid is the product of this fixation, not the acceptor.

    Remember this

    In C4 plants PEP (3C) accepts CO₂ in mesophyll, OAA (4C) is the first product, and RuBP works later in bundle sheath.

  5. Question 5 · Hard

    Read these statements on the Z-scheme. A. The electron lost by P680 is replaced by electrons from the splitting of water. B. Electrons passing from PS II to PS I move uphill on the redox scale. C. Light must be absorbed by both PS II and PS I for NADPH to form. D. Non-cyclic flow forms NADPH but no ATP. Which of these are correct?

    1. AB and D only
    2. BA, C and D only
    3. CA and C only
    4. DA, B and C only
    Show answer

    Answer: C · A and C only

    Water splitting fills the hole left in P680, and both photosystems must be excited for electrons to reach NADP⁺. Travel from PS II to PS I is downhill on the redox scale, so B fails. Non-cyclic flow forms both ATP and NADPH, so D fails.

    • A: A and C are true, and neither B nor D is correct.
    • B: D is false, because non-cyclic flow also makes ATP.
    • D: B is false, because the chain between the photosystems runs downhill.

    Remember this

    Excitation lifts electrons uphill; the chain between PS II and PS I runs downhill and supports ATP synthesis.

  6. Question 6 · Medium

    Given below are two statements: Statement I: Cyclic photophosphorylation forms both ATP and NADPH + H⁺. Statement II: The stroma lamellae lack PS II and NADP reductase. Choose the correct answer.

    1. AStatement I is incorrect but Statement II is correct
    2. BStatement I is correct but Statement II is incorrect
    3. CBoth Statement I and Statement II are correct
    4. DBoth Statement I and Statement II are incorrect

    Answer and explanation in LearnFree account

  7. Question 7 · Easy

    What does cyclic photophosphorylation produce?

    1. AATP only
    2. BATP and NADPH + H⁺
    3. CNADPH + H⁺ only
    4. DATP, NADPH + H⁺ and O₂

    Answer and explanation in LearnFree account

  8. Question 8 · Medium

    Match List-I with List-II. List-I (scientist) A. Julius von Sachs B. Jan Ingenhousz C. Cornelius van Niel D. Joseph Priestley List-II (contribution) I. Hydrogen from an oxidisable compound reduces CO₂, inferred from sulphur bacteria II. Glucose is made in green parts and is usually stored as starch III. Plants restore air spoiled by a candle or a mouse IV. Sunlight is needed, shown by bubbles on green parts of an aquatic plant

    1. AA-II, B-III, C-IV, D-I
    2. BA-I, B-IV, C-II, D-III
    3. CA-IV, B-II, C-I, D-III
    4. DA-II, B-IV, C-I, D-III

    Answer and explanation in LearnFree account

  9. Question 9 · Hard

    A well-watered C3 leaf at its optimum temperature receives 40% of full sunlight in air containing about 0.03% CO₂. Going by Blackman's law of limiting factors, which change will raise its photosynthesis most?

    1. ARaising the temperature well above the optimum
    2. BGiving the plant more water
    3. CRaising CO₂ to 0.05%
    4. DDoubling the light to 80% of full sunlight

    Answer and explanation in LearnFree account

  10. Question 10 · Hard

    Given below are two statements: Statement I: C4 plants show no photorespiration because the breakdown of a C4 acid raises the CO₂ level around RuBisCO in the bundle sheath. Statement II: In C4 plants, the Calvin cycle runs in the mesophyll cells. Choose the correct answer.

    1. AStatement I is incorrect but Statement II is correct
    2. BStatement I is correct but Statement II is incorrect
    3. CBoth Statement I and Statement II are incorrect
    4. DBoth Statement I and Statement II are correct

    Answer and explanation in LearnFree account

  11. Question 11 · Medium

    Tomato and bell pepper give higher yields in greenhouses enriched with CO₂. Which fact explains this?

    1. AThese C4 crops saturate at about 360 μL L⁻¹ of CO₂
    2. BExtra CO₂ makes these crops need more light than full sunlight
    3. CExtra CO₂ stops the closing of their stomata
    4. DThese C3 crops keep raising their photosynthesis at CO₂ levels above the atmospheric level

    Answer and explanation in LearnFree account

  12. Question 12 · Medium

    Which of the following does NOT help to build the proton gradient across the thylakoid membrane?

    1. AMovement of protons through CF₀ into the stroma
    2. BTransfer of protons from stroma to lumen during electron transport
    3. CSplitting of water on the lumen side of the membrane
    4. DRemoval of stromal protons when NADP reductase reduces NADP⁺

    Answer and explanation in LearnFree account

  13. Question 13 · Medium

    Consider these statements on the C4 pathway. A. Bundle sheath cells have thick walls and no intercellular spaces. B. RuBisCO is abundant in the mesophyll cells. C. PEP carboxylase is present in the mesophyll cells. D. A C4 acid releases CO₂ in the bundle sheath cells. Which of these are correct?

    1. AB and C only
    2. BA and B only
    3. CA, B and D only
    4. DA, C and D only

    Answer and explanation in LearnFree account

  14. Question 14 · Medium

    Arrange the following in the order an electron meets them during non-cyclic flow, starting from its source. A. Reaction centre P700 B. Water C. Cytochromes of the electron transport chain D. Reaction centre P680

    1. AB, A, C, D
    2. BD, C, B, A
    3. CB, D, C, A
    4. DD, B, C, A

    Answer and explanation in LearnFree account

  15. Question 15 · Easy

    In Engelmann's experiment, a prism split the light falling on the alga Cladophora kept in a suspension of aerobic bacteria. In which regions of the spectrum did the bacteria collect most?

    1. AYellow and orange
    2. BGreen and yellow
    3. CGreen and red
    4. DBlue and red

    Answer and explanation in LearnFree account

  16. Question 16 · Hard

    Why can the stroma lamellae carry out only cyclic electron flow?

    1. ATheir membranes lack PS I and ATP synthase
    2. BTheir membranes lack PS II and NADP reductase
    3. CThey are surrounded by stroma that has no NADP⁺
    4. DThey lack chlorophyll a and contain only accessory pigments

    Answer and explanation in LearnFree account

  17. Question 17 · Easy

    Working on purple and green sulphur bacteria, who inferred that the oxygen released by green plants comes from water rather than from carbon dioxide?

    1. AT.W. Engelmann
    2. BJoseph Priestley
    3. CCornelius van Niel
    4. DJulius von Sachs

    Answer and explanation in LearnFree account

  18. Question 18 · Hard

    When the action spectrum of photosynthesis is laid over the absorption spectrum of chlorophyll a, some photosynthesis occurs at wavelengths that chlorophyll a absorbs poorly. Which explains this?

    1. APhotorespiration supplies extra ATP at those wavelengths
    2. BChlorophyll a changes its absorption peak at different light intensities
    3. CThose wavelengths drive the Calvin cycle directly without the light reaction
    4. DAccessory pigments absorb those wavelengths and pass the energy to chlorophyll a

    Answer and explanation in LearnFree account

  19. Question 19 · Easy

    Inside a chloroplast, the enzymes that convert CO₂ into sugar are located in the

    1. AGrana membranes
    2. BStroma
    3. CIntermembrane space
    4. DThylakoid lumen

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  20. Question 20 · Medium

    Fixing three molecules of CO₂ through the Calvin cycle requires how much ATP and NADPH?

    1. A9 ATP and 6 NADPH
    2. B18 ATP and 12 NADPH
    3. C3 ATP and 2 NADPH
    4. D6 ATP and 9 NADPH

    Answer and explanation in LearnFree account

  21. Question 21 · Medium

    Given below are two statements: Statement I: In photorespiration, RuBisCO combines RuBP with O₂ to give two molecules of PGA. Statement II: Photorespiration yields ATP and NADPH for the plant. Choose the correct answer.

    1. ABoth Statement I and Statement II are correct
    2. BStatement I is incorrect but Statement II is correct
    3. CBoth Statement I and Statement II are incorrect
    4. DStatement I is correct but Statement II is incorrect

    Answer and explanation in LearnFree account

  22. Question 22 · Medium

    Match List-I with List-II. List-I (Component) A. P680 B. P700 C. Water-splitting complex D. ATP synthase List-II (Feature) I. Reaction centre chlorophyll a of PS I II. Reaction centre chlorophyll a of PS II III. CF₀ embedded in the membrane and CF₁ projecting into the stroma IV. Linked to PS II on the inner side of the thylakoid membrane

    1. AA-I, B-II, C-IV, D-III
    2. BA-II, B-I, C-IV, D-III
    3. CA-II, B-I, C-III, D-IV
    4. DA-II, B-IV, C-I, D-III

    Answer and explanation in LearnFree account

  23. Question 23 · Medium

    Consider these statements about the C4 pathway. A. RuBisCO is present in the bundle sheath cells. B. Mesophyll cells of C4 plants are rich in RuBisCO. C. In mesophyll cells, RuBP is the primary CO₂ acceptor. D. The first stable product of CO₂ fixation is the 4-carbon oxaloacetic acid. Which statements are correct?

    1. AC and D only
    2. BA, C and D only
    3. CA and D only
    4. DA, B and D only

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  24. Question 24 · Easy

    What is the first stable product of CO₂ fixation in the Calvin cycle?

    1. ARibulose bisphosphate
    2. BOxaloacetic acid
    3. C3-phosphoglyceric acid
    4. DPhosphoenol pyruvate

    Answer and explanation in LearnFree account

  25. Question 25 · Medium

    Arrange these steps of the C4 pathway in the correct sequence. A. A C4 acid is decarboxylated in the bundle sheath cells, releasing CO₂ B. PEP carboxylase fixes CO₂ in mesophyll cells to form oxaloacetic acid C. A C4 acid such as malic acid is transported to the bundle sheath cells D. The released CO₂ enters the Calvin cycle Choose the correct order.

    1. AB, A, C, D
    2. BC, B, A, D
    3. CB, C, A, D
    4. DB, C, D, A

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